Antibody Clonality:
Polyclonal
Applications:- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
For short periods of storage (days) store at 4˚C. For longer periods of storage, store TAF15 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
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This product comes from:
United States.
Typical lead time:
14-21 working days.
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Further Information
TAF15, Npl3, RBP56, TAF2N, TAFII68
TAF15 antibody can be used for detection of TAF15 by ELISA at 1:312500. TAF15 antibody can be used for detection of TAF15 by western blot at 3.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. TAF15 encodes a subunit of TFIID present in a subset of TFIID complexes. Translocations involving chromosome 17 and chromosome 9, where the gene for the nuclear receptor CSMF is located, result in a gene fusion product that is an RNA binding protein associated with a subset of extraskeletal myxoid chondrosarcomas.
- Lee, H.J., et al., (2004) FEBS Lett. 564 (1-2), 188-198.
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
batch dependent
Unconjugated
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human TAF15.
8148
TAF15 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 68kDa
TAF15
Homo sapiens
Liquid
PREDICTED MOLECULAR WEIGHT:
62 kDa
NP_631961
21327701
Antibody is purified by protein A chromatography method.
Transcription ,RNA Binding
Q92804
Optimal dilutions for each application to be determined by the researcher.