ProSci

Orexin Receptor 1 Antibody

Product Code:
 
PSI-45-069
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
1 / 1
45-069 (0.1ug/ml) staining of Human Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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45-069 (0.1ug/ml) staining of Human Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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CodeSizePrice
PSI-45-069-0.1mg0.1mg£626.00
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Further Information

Additional Names:
HCRTR1, OX1R, hypocretin (orexin) receptor 1, orexin receptor, orexin receptor 1, orexin receptor-1, hypocretin receptor 1, hypocretin receptor-1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 48+35kDa band observed in Human Brain lysates (calculated MW of 47.5kDa according to NP_001516.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Silveyra et al, Am J Physiol Endocrinol Metab. 2007 Oct;293(4):E977-85; PMID: 17638707). Recommended concentration: 0.1-0.3ug/ml.
Background References:
  • Petersen A, Gil J, Maat-Schieman ML, Bjorkqvist M, Tanila H, Araujo IM, Smith R, Popovic N, Wierup N, Norlen P, Li JY, Roos RA, Sundler F, Mulder H, Brundin P. Orexin loss in Huntington's disease. Hum Mol Genet. 2005 Jan 1;14(1):39-47. Epub 2004 Nov 03.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Sheep
Immunogen:
The immunogen for this antibody is: C-RNWKRPSDQLGD
NCBI Gene ID #:
3061
NCBI Official Name:
hypocretin (orexin) receptor 1
NCBI Official Symbol:
HCRTR1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_001516.1
Protein GI Number:
4557637
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Homeostasis ,Obesity
SPECIFICITY:
.
Swissprot #:
O43613