ProSci

COX-1 Antibody

Product Code:
 
PSI-45-124
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
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45-124 (2ug/ml) staining of U937 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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45-124 Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing Golgi and vesicle staining. The nuclear stain is
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45-124 (2ug/ml) staining of U937 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-124 Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing Golgi and vesicle staining. The nuclear stain is

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PSI-45-124-0.1mg0.1mg£626.00
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Further Information

Additional Names:
PTGS1, COX1, COX3, PHS1, PCOX1, PGHS1, PTGHS, PGG/HS, PGHS-1, prostaglandin-endoperoxide synthase 1 (prostaglandin G/H synthase and cyclooxygenase), prostaglandin G/H synthase and cyclooxygenase, RP11-542K23.6
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 60kDa and 70-75kDa bands observed in lysates of cell line U937 and approx 70-75kDa band observed in Mouse Kidney and Skeletal Muscle lysates (calculated MW of 64.5kDa according to Human NP_542158.1 and 68.7kDa Human NP_000953.2, and 69.0kDa according to Mouse NP_032995.1). The observed molecular weights correspond to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.5-2ug/ml.Immunofluorescence: Strong expression of the protein seen in the Golgi apparatus and vesicles of NIH3T3 cells. Recommended concentration: 5ug/ml.
Background References:
  • Yokoyama C, Tanabe T. Cloning of human gene encoding prostaglandin endoperoxide synthase and primary structure of the enzyme. Biochem Biophys Res Commun. 1989 Dec 15;165(2):888-94.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-QDDGPAVERPSTEL
NCBI Gene ID #:
5742
NCBI Official Name:
prostaglandin-endoperoxide synthase 1 (prostaglandin G/H synthase and cyclooxygenase)
NCBI Official Symbol:
PTGS1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_000953.2, NP_542158.1
Protein GI Number:
18104967
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Neuroscience ,Cancer
SPECIFICITY:
This antibody is expected to recognise both human isoforms of this protein according to NP_000953.2 and NP_542158.1.
Swissprot #:
P23219