Further Information
FTCD, LCHC1, formiminotransferase cyclodeaminase, formimidoyltransferase cyclodeaminase, LC1 autoantigen
Peptide ELISA: antibody detection limit dilution 1:64000.
Western Blot:Approx 60kDa band observed in Human Liver lysates and approx. 55-60kDa in Mouse and Pig Liver lysates, and also in preliminary testing of Rat Liver, Human Kidney and U2OS lysates (calculated MW of 58.9kDa according to Human NP_006648.1, Mouse NP_543121.1 and Pig NP_999440.1). Recommended concentration: 0.03-0.3ug/ml. Primary incubation 1 hour at room temperature.
Immunohistochemistry:In paraffin embedded Human Liver shows distinct staining of membranes and cytoplasm in a selection of hepatocytes. Recommended concentration: 3-5ug/ml.
Immunofluorescence: Strong expression of the protein seen in the cytoplasm, membranes and plasma membranes of HeLa and in the plasma membranes of HepG2 cells. Recommended concentration: 10ug/ml.
Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.
- Bloom GS, Brashear TA. A novel 58- kDa protein associates with the Golgi apparatus and microtubules. J Biol Chem. 1989 Sep 25;264(27):16083-92.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
500 ug/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Expected Species Reactivity based on sequence homology: Rat, Cow
The immunogen for this antibody is: CLREQGRGKDQPGRL
10841
formimidoyltransferase cyclodeaminase
FTCD
Homo sapiens
Liquid
NP_006648.1, NP_996848.1, NP_001307341.1
11140815
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Homeostasis
Variants (NP_006648.1; NP_996848.1) encode the same protein.
O95954