Further Information
KEAP1, kelch-like ECH-associated protein 1, INrf2, KIAA0132, KLHL19, MGC10630, MGC1114, MGC20887, MGC4407, MGC9454, cytosolic inhibitor of Nrf2, kelch-like protein 19
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 75kDa band observed in lysates of cell lines HeLa and NIH3T3 (calculated MW of 69.7kDa according to Human NP_036421.2 and of 69.6kDa according to Mouse NP_057888.1). Recommended concentration: 0.2-0.6ug/ml.Immunohistochemistry:In paraffin embedded Human Cerebellum shows textured cytoplasm staining in Purkinje cell and processes. Recommended concentration, 3-5ug/ml.Immunofluorescence:?Strong expression of the protein seen in the nucleus and cytoplasm of HeLa cells. Recommended concentration: 5ug/ml.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
500 ug/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Expected Species Reactivity based on sequence homology: Rat, Dog, Pig, Cow
The immunogen for this antibody is: C-EVTPSQHGNRTFS
9817
kelch-like ECH-associated protein 1
KEAP1
Homo sapiens
Liquid
NP_036421.2
22027642
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Reported variants represent identical protein: NP_036421.2, NP_987096.1
Q14145