Host Type:
Caprine (Goat)
Antibody Clonality:
Polyclonal
Applications:- Enzyme Immunoassay (EIA)
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
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This product comes from:
United States.
Typical lead time:
14-21 working days.
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Further Information
JUP, GAMMA-CATENIN, gamma-catenin, junction plakoglobin, DP3, PDGB, PKGB, DPIII, ARVD12, CTNNG, OTTHUMP00000164735, catenin (cadherin-associated protein), gamma (80kD), catenin (cadherin-associated protein), gamma 80kDa
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 80kDa band observed in lysates of cell lines A431 and NIH3T3 and in Rat Eye lysates (calculated MW of 81.7kDa according to Human NP_002221.1 and Mouse NP_034723.1). Recommended concentration: 0.1-0.3ug/ml.Immunohistochemistry:Paraffin embedded Human Heart. Recommended concentration: 5ug/ml.Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
- Knudsen KA, Wheelock MJ. Plakoglobin, or an 83-kD homologue distinct from beta-catenin, interacts with E-cadherin and N-cadherin. J Cell Biol. 1992 Aug;118(3):671-9.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
500 ug/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Expected Species Reactivity based on sequence homology: Dog, Pig
The immunogen for this antibody is: C-GLRPPYPTADHMLA
3728
junction plakoglobin
JUP
Homo sapiens
Liquid
NP_002221.1, NP_068831.1
4504811
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
This antibody is expected to recognise both reported isoforms, as represented by NP_002221.1 and NP_068831.1.
P14923