ProSci

4-1BBL Antibody

Product Code:
 
PSI-38-124
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
4-1BBL antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze
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This antibody stained formalin-fixed, paraffin-embedded sections of human colon/rectum adenocarcinoma. The recommended concentration is 0.25 mg/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Proteinase K antigen retrieval is recommended. Optimal concentrations and conditions may vary.
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This antibody stained formalin-fixed, paraffin-embedded sections of human colon/rectum adenocarcinoma. The recommended concentration is 0.25 mg/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Proteinase K antigen retrieval is recommended. Optimal concentrations and conditions may vary.

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PSI-38-124-0.1mg0.1mg£524.00
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Further Information

Additional Names:
CD137L, 4-1BB-LTumor necrosis factor ligand superfamily member 9, 4-1BB ligand, 4-1BBL
Application Note:
ELISA:
Indirect:
To detect h4-1BBL by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant h4-1BBL.

Sandwich
To detect h4-1BBL by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Human 4-1BBL as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant h4-1BBL.

Western Blot:
To detect h4-1BBL by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant h4-1BBL is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant h4-1BBL (human 4-1BBLigand). Human 4-1BBL specific antibody was purified by affinity chromatography employing immobilized h4-1BBL matrix.
NCBI Gene ID #:
8744
NCBI Official Name:
tumor necrosis factor (ligand) superfamily, member 9
NCBI Official Symbol:
TNFSF9
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
P41273
Protein GI Number:
728739
Research Area:
Antibody Pairs
Swissprot #:
P41273
User NOte:
Centrifuge vial prior to opening.