Host Type:
Caprine (Goat)
Antibody Clonality:
Polyclonal
Applications:- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
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![45-046 (0.3ug/ml) staining of HeLa (A) and HepG2 (B) cell lysate (RIPA buffer, 30ug total protein per lane). Detected by chemiluminescence. 45-046 (0.3ug/ml) staining of HeLa (A) and HepG2 (B) cell lysate (RIPA buffer, 30ug total protein per lane). Detected by chemiluminescence.](https://www.prosci-inc.com/static-images/45-046_1.jpg)
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![](https://www.prosci-inc.com/static-images/45-046_2.jpg)
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![](https://www.prosci-inc.com/static-images/45-046_3.jpg)
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![45-046 (2.5ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. <strong>This data is from a previous batch, not on sale.</strong> 45-046 (2.5ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. <strong>This data is from a previous batch, not on sale.</strong>](https://www.prosci-inc.com/static-images/45-046_4.jpg)
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![45-046 staining (0.3ug/ml) of Human Kidney lysate (RIPA buffer, 30ug total protein per lane). Detected by chemiluminescence. 45-046 staining (0.3ug/ml) of Human Kidney lysate (RIPA buffer, 30ug total protein per lane). Detected by chemiluminescence.](https://www.prosci-inc.com/static-images/45-046_5.jpg)
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![45-046 Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue) 45-046 Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue)](https://www.prosci-inc.com/static-images/45-046_6.jpg)
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This product comes from:
United States.
Typical lead time:
14-21 working days.
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- Further Information
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Further Information
DAP3, death associated protein 3, DAP-3, DKFZp686G12159, MGC126058, MGC126059, MRP-S29, MRPS29, bMRP-10, death-associated protein 3, mitochondrial 28S ribosomal protein S29
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 40kDa band observed in lysates of cell lines HeLa and HepG2 and in Human Kidney lysates (calculated MW of 41kDa according to NP_001186779.1 and NP_001186780). Recommended concentration: 0.1-1ug/ml. Primary incubated 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in the nuclei of MCF7 cells. Recommended concentration: 10ug/ml.
- Kissil JL, Deiss LP, Bayewitch M, Raveh T, Khaspekov G, Kimchi A. Isolation of DAP3, a novel mediator of interferon-gamma-induced cell death. J Biol Chem. 1995 Nov 17;270(46):27932-6.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
500 ug/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
The immunogen for this antibody is: NPSLLERHCAYL
7818
death associated protein 3
DAP3
Homo sapiens
Liquid
NP_387506.1, NP_001186779.1, NP_001186780.1
16905526
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Apoptosis
This antibody is expected to recognise isoform 1 (NP_387506.1), isoform 2 (NP_001186779.1) and isoform 3 (NP_001186780.1). Reported variants represent identical protein (NP_387506.1; NP_004623.1; NP_001186778.1).
P51398