ProSci

PBEF1 Antibody

Product Code:
 
PSI-29-726
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Canine (dog)
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
For short periods of storage (days) store at 4˚C. For longer periods of storage, store PBEF1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
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Antibody used in WB on Human Jurkat 1.25 ug/ml.
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Antibody used in WB on Human Jurkat 1.25 ug/ml.

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PSI-29-726-100ul100ul£609.00
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This product comes from: United States.
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Further Information

Additional Names:
PBEF1, 1110035O14Rik, DKFZP666B131, MGC117256, NAMPT, PBEF, VF, PBEF1, VISFATIN
Application Note:
PBEF1 antibody can be used for detection of PBEF1 by ELISA at 1:312500. PBEF1 antibody can be used for detection of PBEF1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Background:
PBEF1 catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, one step in the biosynthesis of nicotinamide adenine dinucleotide. The protein is an adipokine that is localized to the bloodstream and has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It also activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity.
Background References:
  • Chen, M.P., (2006) J. Clin. Endocrinol. Metab. 91 (1), 295-299.
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PBEF1.
NCBI Gene ID #:
10135
NCBI Official Name:
nicotinamide phosphoribosyltransferase
NCBI Official Symbol:
NAMPT
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
54 kDa
Protein Accession #:
NP_005737
Protein GI Number:
5031977
Purification:
Antibody is purified by protein A chromatography method.
Research Area:
Signal Transduction
Swissprot #:
P43490
User NOte:
Optimal dilutions for each application to be determined by the researcher.