Antibody Clonality:
Monoclonal
Applications:- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Storage:
BTK monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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![Western blot analysis using BTK monoclonal antibody against truncated BTK recombinant protein and K562 cell lysate. Western blot analysis using BTK monoclonal antibody against truncated BTK recombinant protein and K562 cell lysate.](https://www.prosci-inc.com/static-images/32-123.jpg)
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![](https://www.prosci-inc.com/static-images/32-123a.jpg)
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This product comes from:
United States.
Typical lead time:
14-21 working days.
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Further Information
AGMX1, ATK, BPK, Agammaglobulinemia tyrosine kinase, AT, XLA, IMD1, AGMX1, PSCTK1
Western Blot:1:500 - 1:2,000
IHC(P):1:500 - 1:2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Brutons tyrosine kinase (BTK) is a member of the BTK/Tec family of cytoplasmic tyrosine kinases.All members of the family contain SH3 and SH2 domains and, with the exception of Txk and Dsrc28C, also contain a pleckstrin homology (PH) and a Tec homology (TH) domain in their amino termini.BTK plays an important role in B cell development. Activation of B cells by various ligands is accompanied by BTK membrane translocation mediated by its PH domain binding to phosphatidylinositol-3,4,5-trisphosphate. The membrane located BTK is active and associated with transient phosphorylation of two tyrosine residues, Tyr551 and Tyr223. Tyr551 in the activation loop is transphosphorylated by the Src family tyrosine kinase, leading to autophosphorylation at Tyr223 within the SH3 domain, which is necessary for full activation.
- Yamada, N., et al. Biochem. Biophys. Res. Commun. 192: 231-240.
- Thomas, J.D., et al. 1993. Science. 261: 355-358.
- Tamagnone, L., et al. Oncogene 9: 3683-3688.
Ascitic fluid containing 0.03% sodium azide.
batch dependent
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Ni-NTA purified truncated recombinant BTK expressed in E. Coli strain BL21 (DE3).
695
Bruton agammaglobulinemia tyrosine kinase
BTK
Homo sapiens
Q06187
547759
Signal Transduction ,Innate Immunity
Q06187
Optimal dilutions for each application to be determined by the researcher.