ProSci

Trim5a Monoclonal Antibody

Product Code:
 
PSI-32-231
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Mouse
Antibody Isotype:
 
IgG1
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
3B11H2
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
Trim5? monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis using Trim5a monoclonal antibody against truncated Trim5αrecombinant protein.
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Western blot analysis using Trim5a monoclonal antibody against truncated Trim5αrecombinant protein.

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PSI-32-231-0.1mL0.1mL£626.00
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Further Information

Additional Names:
C20orf99C20orf99
Application Note:
Western Blot:Dilution 1:1,000 - 1:5,000
IHC(P):Dilution 1:100 - 1:400
IHC(F):Dilution 1:200 - 1:400
Determining optimal working dilutions by titration test.
Background:
TRIM5-alpha is a protein that is found in the cells of many mammals and fends of various retrovirus infections. It protects monkeys from infection with HIV-1, and humans from infection with some other viruses. If a retrovirus has entered a cell, it needs to shed its capsid in order to reversely transcribe its genes, so that they can be expressed by the host cell. It is believed that TRIM5α, which is present in the cytoplasm, somehow recognizes the capsid and blocks its shedding, thereby stopping the virus in its tracks. It thus represents an intracellular defense completely separate from the rest of the body's immune system.
Background References:
  • Stremlau, M. Nature 2004.427:848-53.
  • Song, B. J Virol. 2005.79(7):3930-7.
Buffer:
Ascitic fluid containing 0.03% sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Ni-NTA purified truncated recombinant human trim5 alpha expressed in E. Coli strain BL21 (DE3).
NCBI Gene ID #:
85364
NCBI Official Name:
zinc finger, CCHC domain containing 3
NCBI Official Symbol:
ZCCHC3
NCBI Organism:
Homo sapiens
Protein Accession #:
Q9NUD5
Protein GI Number:
23396534
Research Area:
Infectious Disease
Swissprot #:
Q9NUD5
User NOte:
Optimal dilutions for each application to be determined by the researcher.