ProSci

P2rx4 Antibody

Product Code:
 
PSI-43-001
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
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43-001 (0.3ug/ml) staining of A549 cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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43-001 (0.3ug/ml) staining of A549 cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

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CodeSizePrice
PSI-43-001-0.1mg0.1mg£626.00
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This product comes from: United States.
Typical lead time: 14-21 working days.
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Further Information

Additional Names:
P2rx4, purinergic receptor P2X, ligand-gated ion channel, 4, P2X4, AI504491, AW555605, D5Ertd444e, ATP receptor, OTTMUSP00000023741, OTTMUSP00000023745, OTTMUSP00000023746, OTTMUSP00000023747, P2X purinoceptor 4, ionotropic purinergic receptor
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 48-50kDa band observed in lysates of cell line A549 (calculated MW of 43.4kDa according to Human NP_002551.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.3-1ug/ml. Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on Mouse and Rat Kidney and Liver for this particular batch.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 cells. Recommended concentration: 10ug/ml.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-ETDSVVSSVTTKAK
NCBI Gene ID #:
5025
NCBI Official Name:
purinergic receptor P2X, ligand-gated ion channel, 4
NCBI Official Symbol:
P2rx4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 60 kDa
Protein Accession #:
NP_035156.2
Protein GI Number:
28416927
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
SPECIFICITY:
.
Swissprot #:
Q99571