ProSci

Syntenin Antibody

Product Code:
 
PSI-46-459
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
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46-459 (0.01ug/ml) staining of Human HEK293 cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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46-459 (0.01ug/ml) staining of Human HEK293 cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

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PSI-46-459-0.1mg0.1mg£626.00
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Further Information

Additional Names:
SDCBP, Syntenin, syndecan binding protein (syntenin), MDA-9, ST1, SYCL, TACIP18 , melanoma differentiation associated protein-9, pro-TGF-alpha cytoplasmic domain-interacting protein 18, scaffold protein Pbp1 syntenin
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 28-30kDa band observed in lysates of cell lines HEK293 and A431 (calculated MW of 32.4kDa according to NP_005616.2 and NP_001007068.1 and of 32.3kDa according to NP_001007070.1 and NP_001007071.1). Recommended concentration: 0.01-0.03?g/ml. Primary incubation was 1 hour. IHC: Paraffin embedded Human Breast. Recommended concentration: 10?g/ml.
Background References:
  • Latysheva N, Muratov G, Rajesh S, Padgett M, Hotchin NA, Overduin M, Berditchevski F. Syntenin-1 is a new component of tetraspanin-enriched microdomains: mechanisms and consequences of the interaction of syntenin-1 with CD63. Mol Cell Biol. 2006 Aug 14;
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: SLEDLKVDKVIQAQ-C
NCBI Gene ID #:
6386
NCBI Official Name:
syndecan binding protein (syntenin)
NCBI Official Symbol:
SDCBP
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_005616.2, NP_001007068.1, NP_001007069, NP_001007070.1, NP_001007071.1
Protein GI Number:
56243522
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognise isoform 1 (NP_005616.2 and NP_001007068.1) and isoform 3 (NP_001007070.1and NP_001007071.1) only.
Swissprot #:
O00560