ProSci

VPS28 Antibody

Product Code:
 
PSI-46-580
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
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46-580 staining (1ug/ml) of Human Testis lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
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46-580 staining (1ug/ml) of Human Testis lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

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CodeSizePrice
PSI-46-580-0.1mg0.1mg£626.00
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Further Information

Additional Names:
VPS28, vacuolar protein sorting 28 (yeast), vacuolar protein sorting 28 homolog (S. cerevisiae), MGC60323, vacuolar protein sorting 28, yeast class E protein Vps28p homolog
Application Note:
Peptide ELISA: antibody detection limit dilution 1:1000.Western Blot:Approx 24kDa band observed in H460, Human Testis and Brain lysates (predicted MW of 25kDa according to NP_057292.1). Recommended for use at 1-3ug/ml.Immunohistochemistry:In paraffin embedded Human Testis, stains acrosomal head caps in round spermatids. Recommended concentration: 10ug/ml.
Background References:
  • Bishop N, Woodman P. TSG101/mammalian VPS23 and mammalian VPS28 interact directly and are recruited to VPS4-induced endosomes. J Biol Chem. 2001 Apr 13;276(15):11735-42.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig
Immunogen:
The immunogen for this antibody is: C-ESAYNAFNRFLHA
NCBI Gene ID #:
51160
NCBI Official Name:
vacuolar protein sorting 28 homolog (S. cerevisiae)
NCBI Official Symbol:
VPS28
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_057292.1
Protein GI Number:
7705885
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognize isoform 1 (NP_057292.1) only.
Swissprot #:
Q9UK41