ProSci

Sirt3 Antibody

Product Code:
 
PSI-42-388
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
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42-388 Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI
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42-388 Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI

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PSI-42-388-0.1mg0.1mg£626.00
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This product comes from: United States.
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Further Information

Additional Names:
Sirt3, sirtuin 3, sirtuin 3 (silent mating type information regulation 2, homolog) 3, 2310003L23Rik, AI848213, Sir2l3, NAD-dependent deacetylase sirtuin-3, OTTMUSP00000027441, OTTMUSP00000027442, OTTMUSP00000027444, OTTMUSP00000027445, SIR2-like protein 3, SIRT3L mitochondrial, mSIR2L3, mitochondrial protein lysine deacetylase, silent mating type information regulation 2, (S.cerevisiae, homolog)-like 3
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 30kDa band observed in Mouse Heart lysates (calculated MW of 28.8kDa according to NP_071878.2). An additional band of unknown identity was also consistently observed at 15kDa. This band was successfully blocked by incubation with the immunizing peptide. Recommended concentration: 1-3ug/ml. Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on Rat Kidney for this particular batch.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of NIH3T3 cells. Recommended concentration: 10ug/ml.

Flow Cytometry: Flow cytometric analysis of NIH3T3 cells. Recommended concentration: 10ug/ml.

Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Human, Rat, Pig
Immunogen:
The immunogen for this antibody is: C-RASGIPASKLVEAH
NCBI Gene ID #:
23410
NCBI Official Name:
sirtuin 3
NCBI Official Symbol:
Sirt3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_071878.2, NP_001171275.1
Protein GI Number:
188035865
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
SPECIFICITY:
This antibody is expected to recognize both reported isoforms (NP_071878.2; NP_001171275.1). Reported variants represent identical proteins: NP_071878.2; NP_001120823.1
Swissprot #:
Q9NTG7