Product Code:
PSI-XP-5191
Antibody Clonality:
Polyclonal
Applications:- Enzyme-Linked Immunosorbent Assay (ELISA)
- Neutralisation
- Western Blot (WB)
Storage:
IL-5 antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-t
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This product comes from:
United States.
Typical lead time:
14-21 working days.
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Further Information
EDF, TRF, IL-5Interleukin-5, B-cell differentiation factor I, IL-5
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hIL-5 (0.45 ng/mL), a concentration of 0.05 -0.09 μg/mL of this antibody is required.
ELISA:
To detect hIL-5 by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIL-5.
Sandwich:
To detect hIL-5 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human IL-5 (XP-5191Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-5.
Western Blot:
To detect hIL-5 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-5 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Interleukin 5 (IL-5) is a cytokine produced primarily by activated T lymphocytes. It exists as an antiparallel disulfide linked homodimeric glycoprotein with 115 amino acid residues in each chain. Known also as EDF (eosinophil differentiating factor), functions predominantly as an eosinophilopoietic factor. Analysis of its crystal structure reveals a novel two domain structure, with each domain showing significant homology to the cytokine fold in GMCSF, MCSF, IL-2, IL-4 and growth hormone. Human and mouse IL-5 have 70% amino acid sequence homology.
batch dependent
Unconjugated
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-5 (human Interleukin-5).
3567
interleukin 5 (colony-stimulating factor, eosinophil)
IL5
Homo sapiens
Lyophilized
P05113
124341
Anti-hIL-5 specific antibody was purified by affinity chromatography employing immobilized hIL-5 matrix.
Immunology,Chemokines & Cytokines,Antibody Pairs, Functional Assays
P05113
Centrifuge vial prior to opening.