ProSci

ELA1 Antibody

Product Code:
 
PSI-26-628
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
For short periods of storage (days) store at 4˚C. For longer periods of storage, store ELA1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
1 / 1
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.
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Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.

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PSI-26-628-100ul100ul£662.00
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Further Information

Additional Names:
ELA1, ELA1
Application Note:
ELA1 antibody can be used for detection of ELA1 by ELISA at 1:62500. ELA1 antibody can be used for detection of ELA1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Background:
Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, pancreatic elastase 1 is not expressed in the pancreas. To date, elastase 1 (ELA1) expression has only been detected in skin keratinocytes. Clinical literature that describes human elastase 1 activity in the pancreas is actually referring to elastase 2A.Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, pancreatic elastase 1 is not expressed in the pancreas. To date, elastase 1 expression has only been detected in skin keratinocytes. Clinical literature that describes human elastase 1 activity in the pancreas is actually referring to elastase 2A. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-867 BC069454.1 1-867 868-952 AF120493.1 868-952
Background References:
  • Hahn, J.U., (2008) Pancreas 36 (3), 274-278.
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ELA1.
NCBI Gene ID #:
1990
NCBI Official Name:
chymotrypsin-like elastase family, member 1
NCBI Official Symbol:
CELA1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
28 kDa
Protein Accession #:
NP_001962
Protein GI Number:
58331209
Purification:
Antibody is purified by peptide affinity chromatography method.
Swissprot #:
Q9UNI1
User NOte:
Optimal dilutions for each application to be determined by the researcher.