Host Type:
Caprine (Goat)
Antibody Clonality:
Polyclonal
Applications:- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
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![45-282 staining (0.5ug/ml) of U937 lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence. 45-282 staining (0.5ug/ml) of U937 lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.](https://www.prosci-inc.com/static-images/45-282_1.jpg)
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![](https://www.prosci-inc.com/static-images/45-282_2.jpg)
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This product comes from:
United States.
Typical lead time:
14-21 working days.
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- Further Information
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Further Information
ARIH1, HHARI, ARI, HARI, UBCH7BP, ariadne homolog, ubiquitin-conjugating enzyme E2 binding protein, 1 (Drosophila), ariadne homolog, ariadne, Drosophila, homolog of, DKFZp686O13120, FLJ20329, FLJ93118, ariadne ubiquitin-conjugating enzyme E2 binding protein homolog 1
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 65kDa band seen in U937 lysate [Predicted MW of approx. 61kDa according to NP_005735]. Recommended for use at 0.2-1ug/ml.Immunohistochemistry:Paraffin embedded Human Adrenal Gland. Recommended concentration: 5ug/ml.
- Moynihan TP, Ardley HC, Nuber U, Rose SA, Jones PF, Markham AF, Scheffner M, Robinson PA. The ubiquitin-conjugating enzymes UbcH7 and UbcH8 interact with RING finger/IBR motif-containing domains of HHARI and H7-AP1. J Biol Chem. 1999 Oct 22;274(43):30963-8.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
500 ug/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Expected Species Reactivity based on sequence homology: Mouse, Cow
The immunogen for this antibody is: C-HEGYEKDLWEYIED
25820
ariadne RBR E3 ubiquitin protein ligase 1
ARIH1
Homo sapiens
Liquid
NP_005735.2
187761373
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Cancer
.
Q9Y4X5