ProSci

CDEP Antibody

Product Code:
 
PSI-45-385
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
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45-385 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (b
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45-385 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (b

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PSI-45-385-0.1mg0.1mg£626.00
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Further Information

Additional Names:
FARP1, FERM, RhoGEF (ARHGEF) and pleckstrin domain protein 1 (chondrocyte-derived), CDEP, chondrocyte-derived ezrin-like protein, RP11-111L24.1, MGC87400, PLEKH2, FERM, RhoGEF, and pleckstrin domain protein 1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments showed an approx 110-120kDa band in Human Frontal Cortex lysates (calculated MW of 119kDa according to NP_ 005757.1).. An additional band was also consistently observed at 40kDa and was successfully blocked by incubation with the immunizing peptide. Recommended concentration: 0.3-1ug/ml. Primary incubation 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U2OS and HeLa cells. Recommended concentration: 10ug/ml.

Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.

Background References:
  • Koyano Y, Kawamoto T, Shen M, Yan W, Noshiro M, Fujii K, Kato Y. Molecular cloning and characterization of CDEP, a novel human protein containing the ezrin-like domain of the band 4.1 superfamily and the Dbl homology domain of Rho guanine nucleotide exchange factors. Biochem Biophys Res Commun. 1997 Dec 18;241(2):369-75.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Cow
Immunogen:
The immunogen for this antibody is: GEIEQRPTPGSRL-C
NCBI Gene ID #:
10160
NCBI Official Name:
FERM, RhoGEF (ARHGEF) and pleckstrin domain protein 1 (chondrocyte-derived)
NCBI Official Symbol:
FARP1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_005757.1, NP_001001715.2, NP_001273768.1
Protein GI Number:
5031633
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
Swissprot #:
Q9Y4F1