ProSci

HNF4A Antibody

Product Code:
 
PSI-45-740
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
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45-740 (0.1ug/ml) staining of HepG2 lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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45-740 (0.1ug/ml) staining of HepG2 lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

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PSI-45-740-0.1mg0.1mg£626.00
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This product comes from: United States.
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Further Information

Additional Names:
HNF4A, TCF, HNF4, NR2A1, TCF14, HNF4a7, HNF4a8, HNF4a9, NR2A21, hepatocyte nuclear factor 4, alpha, HNF4-alpha, transcription factor-14, FLJ39654, HNF4alpha, MODY, MODY1, HNF4alpha10/11/12, hepatic nuclear factor 4 alpha
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 55kDa and 48kDa bands observed in Mouse Small Intestine and in lysates of cell line HepG2 (calculated MW of 52.8kDa according to Human NP_000448.3 , 46.6kDa according to Human NP_849181.1, 52.7kDa according to Mouse NP_032287.2 and 49.9kDa according to Mouse NP_001299835.1). Recommended concentration: 0.1-0.5ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Small Intestine. Recommended concentration: 2ug/ml.
Background References:
  • Damcott CM, Hoppman N, Ott SH, Reinhart LJ, Wang J, Pollin TI, O'Connell JR, Mitchell BD, Shuldiner AR. Polymorphisms in both promoters of hepatocyte nuclear factor 4-alpha are associated with type 2 diabetes in the Amish. Diabetes. 2004 Dec;53(12):3337-41.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Dog, Pig
Immunogen:
The immunogen for this antibody is: RLSKTLVDMDMADY-C
NCBI Gene ID #:
3172
NCBI Official Name:
hepatocyte nuclear factor 4, alpha
NCBI Official Symbol:
HNF4A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_849180.1, NP_000448.3, NP_849181.1
Protein GI Number:
31077207
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognise the reported isoforms a, b and c (NP_849180.1; NP_000448.3; NP_849181.1 resp.).
Swissprot #:
P41235