ProSci

HSD11B1 Antibody

Product Code:
 
PSI-45-748
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
1 / 1
45-748 (0.3ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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45-748 (0.3ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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PSI-45-748-0.1mg0.1mg£626.00
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This product comes from: United States.
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Further Information

Additional Names:
HSD11B1, HDL, 11-DH, HSD11L, MGC13539, 11-beta-HSD1, hydroxysteroid (11-beta) dehydrogenase 1, corticosteroid 11-beta-dehydrogenase isozyme 1, HSD11, HSD11B, SDR26C1, 11-beta-hydroxysteroid dehydrogenase 1, OTTHUMP00000034650, short chain dehydrogenase/reductase family 26C, member 1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 35kDa band observed in Human Liver lysates (calculated MW of 32.4kDa according to NP_005516.1 and NP_861420.1). Recommended concentration: 0.3-1ug/ml.
Background References:
  • Paterson JM, Morton NM, Fievet C, Kenyon CJ, Holmes MC, Staels B, Seckl JR, Mullins JJ. Metabolic syndrome without obesity: Hepatic overexpression of 11beta-hydroxysteroid dehydrogenase type 1 in transgenic mice. Proc Natl Acad Sci USA. 2004 May 4;101(18):7088-93. Epub 2004 Apr 26.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Cow
Immunogen:
The immunogen for this antibody is: CTSYNMDRFINK
NCBI Gene ID #:
3290
NCBI Official Name:
hydroxysteroid (11-beta) dehydrogenase 1
NCBI Official Symbol:
HSD11B1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_005516.1, NP_861420.1
Protein GI Number:
5031765
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognise human HSD11B1 protein. Both NP_005516.1 and NP_861420.1 variants encode the same protein.
Swissprot #:
P28845