ProSci

Importin 8 Antibody

Product Code:
 
PSI-45-771
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
1 / 1
45-771 (4ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining. Similar results were obtained after antigen retrieval at pH9.
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45-771 (4ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining. Similar results were obtained after antigen retrieval at pH9.

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PSI-45-771-0.1mg0.1mg£626.00
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Further Information

Additional Names:
IPO8, importin 8, RANBP8, RAN-binding protein 8, FLJ26580, RAN binding protein 8
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments in lysates of cell line HeLa gave no specific signal but low background (at antibody concentration up to 1ug/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?Immunohistochemistry:In paraffin embedded Human Tonsil shows cytoplam / perinuclear staining in the parenchyma. Recommended concentration, 4-6ug/ml.
Background References:
  • Gorlich D, Dabrowski M, Bischoff FR, Kutay U, Bork P, Hartmann E, Prehn S, Izaurralde E. A novel class of RanGTP binding proteins. J Cell Biol. 1997 Jul 14;138(1):65-80.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-SAFNFGTVPSNN
NCBI Gene ID #:
10526
NCBI Official Name:
importin 8
NCBI Official Symbol:
IPO8
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_006381.2, NP_001177924.1
Protein GI Number:
53759103
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognise isoform 1 (NP_006381.2) and isoform 2 (NP_001177924.1).
Swissprot #:
O15397