Mouse anti Collagen Type IV
Antibody Clonality:
Monoclonal
Target Species:- Bovine (Cattle)
- Human
- Porcine (pig)
Applications:- Flow Cytometry
- Immunohistochemistry (IHC)
- Western Blot (WB)
Storage:
Store at 4°C or in small aliquots at -20°C.
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This product comes from:
Netherlands.
Typical lead time:
7-10 working days.
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Further Information
Applications Description:
1043 is useful for immunoblotting, immunocytochemistry on fixed cells (methanol fixation) and immunohistochemistry on frozen tissues when using a PBS buffer containing 0.1 mM CaCl2 and 0.1 mM MgCl2 .
For immunohistochemistry on paraffin sections the sections should be pretreated with 1 mg/ml pepsin in 0.01 N HCl for 30 minutes at room temperature. Optimal antibody dilution should be determined by titration; 1:50 ? 1:100 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 ? 1:1000 for immunoblotting applications.
The extent of expression of the basement membrane component type IV collagen may be correlated with the degree of differentiation of basement membrane producing neoplasma. Neoplastic cells of invasive carcinomas often lack a continuous basement membrane. In diagnostic histopathology antibodies to type IV collagen may help to distinguish between non-invasive (benign or in situ) and invasive lesions. Furthermore the antiserum is important in the differential diagnosis of bullous lesions in dermatopathology.
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water.
This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
Cancer|Stem cell research
Each vial contains 100 ul 1mg/ml of purified monoclonal antibody in PBS containing 0.09% sodium azide
Each vial contains 100 ul 1mg/ml of purified monoclonal antibody in PBS containing 0.09% sodium azide
1043 is a mouse monoclonal IgG1 antibody derived by fusion of Sp 2/0 mouse myeloma cells with spleen cells from a BABL/c mouse immunized with purified human type IV collagen. The immunogen has been isolated from human placenta.
Does not react with other species
1043 recognizes type IV collagen in the extracellular - basement membranes of normal and pathological human tissues.
P08572, Q01955, P29400, P53420, P02462