Nephrin Antibody
Product Code:
PSI-7979
PSI-7979
Host Type:
Rabbit
Rabbit
Antibody Isotype:
IgG
IgG
Antibody Clonality:
Polyclonal
Polyclonal
Antibody Clone:
4758822
4758822
Regulatory Status:
RUO
RUO
Target Species:
Human
Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Nephrin antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Nephrin antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
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Code | Size | Price |
---|
PSI-7979-0.02mg | 0.02mg | £150.00 |
Quantity:
PSI-7979-0.1mg | 0.1mg | £449.00 |
Quantity:
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This product comes from: United States.
Typical lead time: 14-21 working days.
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Typical lead time: 14-21 working days.
Contact us for more accurate information.
- Further Information
- Documents
- References
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Further Information
Additional Names:
NPHN, NPHS1, Renal glomerulus-specific cell adhesion receptor3
Application Note:
WB: 1-2 μg/mL; IHC: 5 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry in human samples; Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry in human samples; Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
Nephrin is strongly expressed in renal glomeruli and is a member of the immunoglobulin family of cell adhesion molecules. Mutations in the Nephrin gene result in congenital nephrotic syndrome, an autosomal-recessive disorder characterized by massive proteinuria in utero and nephrosis at birth (1). Renal glomeruli allow normal kidneys to filter plasma so that it is very pure. Nephrin is expressed in the podocyte slit-diaphragm of the renal glomeruli in a manner that suggests that Nephrin molecules homodimerize in an anti-parallel fashion similar to cadherin interactions in adherens junctions. Thus, Nephrin may constitute the entire extracellular structure of the slit-diaphragm (2,3).
Background References:
- Kestila et al. Mol. Cell 1998; 1:575-582.
- Tryggvason. J. Am. Soc. Nephrol. 1999; 10:2440-5.
- Tryggvason and Wartiovaara. Curr. Opin. Nephrol. Hypertens. 2001; 10:543-9.
Buffer:
Nephrin antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rat (93%).
Immunogen:
Anti-Nephrin antibody (7979) was raised against a peptide corresponding to 15 amino acids near the center of human Nephrin.
The immunogen is located within amino acids 1100-1150 of Nephrin.
The immunogen is located within amino acids 1100-1150 of Nephrin.
ISOFORMS:
Human Nephrin has 2 isoforms, including isoform 1 (1241aa, 135kD) and isoform 2 (1201aa, 131kD). Mouse Nephrin also has 2 isoforms, including isoform 1 (1256aa, 136kD) and isoform 2 (1242aa, 135kD). Rat Nephrin has 3 isoforms, including isoform 1 (1252aa, 136kD), isoform 2 (1239aa, 135kD) and isoform 3 (1166aa, 127kD). 7979 can detect human and mouse.
NCBI Gene ID #:
4648
NCBI Official Name:
nephrosis 1, congenital, Finnish type (nephrin)
NCBI Official Symbol:
NPHS1
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 125, 137 kDa
Observed: 125 kDa
Observed: 125 kDa
Protein Accession #:
NP_004637
Protein GI Number:
4758822
Purification:
Nephrin antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis
Swissprot #:
O60500
User NOte:
Optimal dilutions for each application to be determined by the researcher.
VALIDATION:
Induced Expression Validation (Figure 6): Nephrin expression detected by anit-Nephrin antibodies was up-regulated by curcumin treatment in type 1 diabetic rats.
Localization Validation (Figure 7): Nephrin detected by anti-Nephrin antibodies is colocalized with PKGIα at the tips of the cell processes in rat podocytes.
Regulated expression validation (Figure 10): Nephrin expression detected by anit-Nephrin antibodies was down-regulated by PEGF treatment (NGP or HGP), which was reversed by the addition of C3 transferase in mouse podocyte cells.
Documents
References
- Li et al. Mesenchymal stem cells protect podocytes from apoptosis induced by high glucose via secretion of epithelial growth factor. Stem Cell Res Ther. 2013;4(5):103.PMID: 24004644
- Soetikno et al. Curcumin decreases renal triglyceride accumulation through AMPK-SREBP signaling pathway in streptozotocin-induced type 1 diabetic rats. J Nutr Biochem. 2013;24(5):796-802.PMID: 22898567
- Piwkowska et al. Insulin increases glomerular filtration barrier permeability through dimerization of protein kinase G type I? subunits. Biochim Biophys Acta. 2013;1832(6):791-804. PMID: 23454089
- Toyama et al. Proteinuria in AMPD2-deficient mice. Genes Cells. 2012;17(1):28-38. PMID: 22212473
- Huang et al. Increased levels of serum pigment epithelium-derived factor aggravate proteinuria via induction of podocyte actin rearrangement. Int Urol Nephrol. 2019;51(2):359-367. PMID: 30536192
- Piwkowska et al. Hydrogen peroxide induces dimerization of protein kinase G type I? subunits and increases albumin permeability in cultured rat podocytes. J Cell Physiol. 2012;227(3):1004-16. PMID: 21520075
- Rogacka et al. Metformin overcomes high glucose-induced insulin resistance of podocytes by pleiotropic effects on SIRT1 and AMPK. Biochim Biophys Acta Mol Basis Dis. 2018;1864(1):115-125. PMID: 29032153