ProSci

MAP2K2 / MEK2 Antibody

Product Code:
 
PSI-43-442
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
1 / 1
43-442 (0.3ug/ml) staining of Daudi (A), Jurkat (B), K562 (C) and Molt4 (D) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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43-442 (0.3ug/ml) staining of Daudi (A), Jurkat (B), K562 (C) and Molt4 (D) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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PSI-43-442-0.1mg0.1mg£626.00
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This product comes from: United States.
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Further Information

Additional Names:
MAP2K2, mitogen-activated protein kinase kinase 2, MAPKK2, MEK2, MKK2, PRKMK2, ERK activator kinase 2, MAP kinase kinase 2, MAPK/ERK kinase 2, dual specificity mitogen-activated protein kinase kinase 2, mitogen-activated protein kinase kinase 2, p45
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 48kDa band observed in lysates of cell lines Daudi, Jurkat, K562 and Molt4 (calculated MW of 44.4kDa according to NP_109587.1). Recommended concentration: 0.1-0.3ug/ml.
Background References:
  • Duhamel S, H?bert J, Gaboury L, Bouchard A, Simon R, Sauter G, Basik M, Meloche S.Sef downregulation by Ras causes MEK1/2 to become aberrantly nuclear localized leading to polyploidy and neoplastic transformation.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-DLKMLTNHTFIKRS
NCBI Gene ID #:
5605
NCBI Official Name:
mitogen-activated protein kinase kinase 2
NCBI Official Symbol:
MAP2K2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 48kDa
Protein Accession #:
NP_109587.1
Protein GI Number:
13489054
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer,Signal Transduction,Cancer Signal Transduction
Swissprot #:
P36507