ProSci

Dcr-1 Antibody

Product Code:
 
PSI-60-630
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
C. Elegans
Application:
 
Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of Dcr-1 antibody in CEM cell line lysates (35ug/lane)
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Western blot analysis of Dcr-1 antibody in CEM cell line lysates (35ug/lane)

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PSI-60-630-400ul400ul£626.00
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Further Information

Additional Names:
Endoribonuclease dcr-1, 3126-, dcr-1
Application Note:
For WB starting dilution is: 1:1000
Background:
The Dcr-1 gene encodes a bidentate ribonuclease that is homologous to E. coli RNAse III. Dcr-1 is required both for RNA interference and for synthesis of small developmental RNAs. Dcr-1 interacts in vivo with RDE-4, a double-stranded RNA (dsRNA) binding protein required for RNAi that interacts with trigger dsRNAs and may function to deliver dsRNAs to Dcr-1 for endonucleolytic processing.
Background References:
  • Duchaine TF, et al. 2006. Cell 124:343-354.
  • Denli AM, et al. 2004. Nature 432:231-235.
  • Tabara H, et al. 2002. Cell 109:861-871.
  • Grishok A, et al. 2001. Cell 106:23-34.
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This Dcr-1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1068-1097 amino acids from the Central region of human Dcr-1.
NCBI Gene ID #:
176138
NCBI Official Name:
Endoribonuclease dcr-1
NCBI Official Symbol:
dcr-1
NCBI Organism:
Caenorhabditis elegans
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
218 kDa
Protein Accession #:
P34529
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Swissprot #:
P34529
User NOte:
Optimal dilutions for each application to be determined by the researcher.