ProSci

PRAK Antibody

Product Code:
 
PSI-62-736
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
1 / 2
Western Blot at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 ug per lane.
2 / 2
Western blot analysis of MAPKAPK5 using rabbit polyclonal MAPKAPK5 Antibody (T182) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MAPKAPK5 gene.
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Western Blot at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis of MAPKAPK5 using rabbit polyclonal MAPKAPK5 Antibody (T182) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MAPKAPK5 gene.

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PSI-62-736-400ul400ul£626.00
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Further Information

Additional Names:
MAP kinase-activated protein kinase 5, MAPK-activated protein kinase 5, MAPKAP kinase 5, MAPKAP-K5, MAPKAPK-5, MK-5, MK5, p38-regulated/activated protein kinase, PRAK, MAPKAPK5, PRAK
Application Note:
For WB starting dilution is: 1:1000
Background:
MAPKAPK5 is a member of the serine/threonine kinase family. In response to cellular stress and proinflammatory cytokines, this kinase is activated through its phosphorylation by MAP kinases including MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta. In vitro, this kinase phosphorylates heat shock protein HSP27 at its physiologically relevant sites.
Background References:
  • New, L., et al., EMBO J. 17(12):3372-3384 (1998).
  • Ni, H., et al., Biochem. Biophys. Res. Commun. 243(2):492-496 (1998).
  • Sudo T.,Biochem. Biophys. Res. Commun. 269:521-525(2000).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This PRAK(MAPKAPK5) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 160-189 amino acids from human PRAK(MAPKAPK5).
NCBI Gene ID #:
8550
NCBI Official Name:
MAP kinase-activated protein kinase 5
NCBI Official Symbol:
MAPKAPK5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
54 kDa
Protein Accession #:
Q8IW41
Protein GI Number:
52000829
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer
Swissprot #:
Q8IW41
User NOte:
Optimal dilutions for each application to be determined by the researcher.