ProSci

CENPH Antibody

Product Code:
 
PSI-63-840
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of CENPH Antibody in Hela cell line lysates (35ug/lane)
2 / 3
Formalin-fixed and paraffin-embedded human testis tissue reacted with CENPH Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
3 / 3
❮❯

Western blot analysis of CENPH Antibody in Hela cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human testis tissue reacted with CENPH Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

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PSI-63-840-400ul400ul£626.00
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Further Information

Additional Names:
Centromere protein H, CENP-H, Interphase centromere complex protein 35, CENPH (HGNC:17268), ICEN35
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
Centromere and kinetochore proteins play a critical role in centromere structure, kinetochore formation, and sister chromatid separation. The protein encoded by this gene colocalizes with inner kinetochore plate proteins CENP-A and CENP-C in both interphase and metaphase. It localizes outside of centromeric heterochromatin, where CENP-B is localized, and inside the kinetochore corona, where CENP-E is localized during prometaphase. It is thought that this protein can bind to itself, as well as to CENP-A, CENP-B or CENP-C. Multimers of the protein localize constitutively to the inner kinetochore plate and play an important role in the organization and function of the active centromere-kinetochore complex.
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This CENPH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 136-162 amino acids from the Central region of human CENPH.
NCBI Gene ID #:
64946
NCBI Official Name:
Centromere protein H
NCBI Official Symbol:
CENPH
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
28 kDa
Protein Accession #:
Q9H3R5
Protein GI Number:
74733576
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer
Swissprot #:
Q9H3R5
User NOte:
Optimal dilutions for each application to be determined by the researcher.