ProSci

ETS1 Antibody

Product Code:
 
PSI-55-366
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
1 / 3
Western Blot at 1:1000 dilution Lane 1: Jurkat whole cell lysates Lane 2: 293T whole cell lysates Lysates/proteins at 20 ug per lane.
2 / 3
Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
3 / 3
❮❯

Western Blot at 1:1000 dilution Lane 1: Jurkat whole cell lysates Lane 2: 293T whole cell lysates Lysates/proteins at 20 ug per lane.
Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

No additional charges, what you see is what you pay! *

CodeSizePrice
PSI-55-366-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT
How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: United States.
Typical lead time: 14-21 working days.
Contact us for more accurate information.
  • Further Information
  • Documents
  • Show All

Further Information

Additional Names:
Protein C-ets-1, p54, ETS1, EWSR2
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50

For IF starting dilution is: 1:10~50
Background:
ETS transcriptions factors, such as ETS1, regulate numerous genes and are involved in stem cell development, cell senescence and death, and tumorigenesis. The conserved ETS domain within these proteins is a winged helix-turn-helix DNA-binding domain that recognizes the core consensus DNA sequence GGAA/T of target genes (summary by Dwyer et al., 2007 [PubMed 17986575]).
Background References:
  • Harris, T.A., et al. Arterioscler. Thromb. Vasc. Biol. 30(10):1990-1997(2010) He, C.F., et al. Lupus 19(10):1181-1186(2010) Russell, L., et al. Cytokine 51(3):217-226(2010) Li, T., et al. Pharm Biol 48(2):161-165(2010) Kunderfranco, P., et al. PLoS ONE(5), E10547 (2010) :
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This ETS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 43-70 amino acids from the N-terminal region of human ETS1.
NCBI Gene ID #:
2113
NCBI Official Name:
Protein C-ets-1
NCBI Official Symbol:
ETS1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
50 kDa
Protein Accession #:
P14921
Protein GI Number:
119641
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,
Swissprot #:
P14921
User NOte:
Optimal dilutions for each application to be determined by the researcher.