ProSci

Cyclin A Antibody

Product Code:
 
PSI-62-797
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
1 / 3
Western blot analysis of anti-CCNA2Pab in K562 cell line lysates (35ug/lane).
2 / 3
Formalin-fixed and paraffin-embedded human hepatocarcinoma with Cyclin A (CCNA2) Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
3 / 3
❮❯

Western blot analysis of anti-CCNA2Pab in K562 cell line lysates (35ug/lane).
Formalin-fixed and paraffin-embedded human hepatocarcinoma with Cyclin A (CCNA2) Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

No additional charges, what you see is what you pay! *

CodeSizePrice
PSI-62-797-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT
How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: United States.
Typical lead time: 14-21 working days.
Contact us for more accurate information.
  • Further Information
  • Documents
  • Show All

Further Information

Additional Names:
Cyclin-A2, Cyclin-A, CCNA2, CCN1, CCNA
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
CCNA2 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. This cyclin binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions.
Background References:
  • Henglein B.,Proc. Natl. Acad. Sci. U.S.A. 91:5490-5494(1994)
  • Wang J.,Nature 343:555-557(1990)
  • Tsang,W.Y.,J. Cell Biol. 178 (4), 621-633 (2007)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This Cyclin A (CCNA2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 51-84 amino acids from the N-terminal region of human Cyclin A (CCNA2).
NCBI Gene ID #:
890
NCBI Official Name:
Cyclin-A2
NCBI Official Symbol:
CCNA2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
49 kDa
Protein Accession #:
P20248
Protein GI Number:
311033358
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer,Cell Cycle
Swissprot #:
P20248
User NOte:
Optimal dilutions for each application to be determined by the researcher.