ProSci

SOD2 Antibody

Product Code:
 
PSI-63-009
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of anti-SOD2 Pab in K562 cell line lysates (35ug/lane)
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Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Western blot analysis of anti-SOD2 Pab in K562 cell line lysates (35ug/lane)
Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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PSI-63-009-400ul400ul£626.00
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Further Information

Additional Names:
Superoxide dismutase [Mn], mitochondrial, SOD2
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
SOD2 is a member of the iron/manganese superoxide dismutase family. It is a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer.
Background References:
  • Fabre,E.E., Am. J. Clin. Nutr. 87 (5), 1504-1512 (2008) Kaewpila,S., Cancer Res. 68 (8), 2781-2788 (2008) Flekac,M., (er) BMC Med. Genet. 9, 30 (2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This SOD2 antibody is generated from rabbits immunized with human recombinant SD protein.
NCBI Gene ID #:
6648
NCBI Official Name:
Superoxide dismutase [Mn], mitochondrial
NCBI Official Symbol:
SOD2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
25 kDa
Protein Accession #:
P04179
Protein GI Number:
134665
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer,Cell Cycle,Obesity,Neuroscience,Signal Transduction
Swissprot #:
P04179
User NOte:
Optimal dilutions for each application to be determined by the researcher.