ProSci

Myeloperoxidase Antibody

Product Code:
 
PSI-55-914
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the detected the Myeloperoxidase protein (arrow).
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Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the detected the Myeloperoxidase protein (arrow).
Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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PSI-55-914-400ul400ul£626.00
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Further Information

Additional Names:
Myeloperoxidase, MPO, Myeloperoxidase, 89 kDa myeloperoxidase, 84 kDa myeloperoxidase, Myeloperoxidase light chain, Myeloperoxidase heavy chain, MPO
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of netrophils. [provided by RefSeq].
Background References:
  • Banerjee, M., et al. Toxicol. Appl. Pharmacol. 249(1):47-54(2010)
  • Shimada, M., et al. Hum. Genet. 128(4):433-441(2010)
  • Nahon, P., et al. Antioxid. Redox Signal. (2010) In press :
  • Wang, Y., et al. J. Huazhong Univ. Sci. Technol. Med. Sci. 30(4):437-442(2010)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This Myeloperoxidase antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 60-89 amino acids from the N-terminal region of human Myeloperoxidase.
NCBI Gene ID #:
4353
NCBI Official Name:
Myeloperoxidase
NCBI Official Symbol:
MPO
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
84 kDa
Protein Accession #:
P05164
Protein GI Number:
129825
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Immunology,Obesity
Swissprot #:
P05164
User NOte:
Optimal dilutions for each application to be determined by the researcher.