ProSci

CD8A Antibody

Product Code:
 
PSI-57-512
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
1 / 3
Western blot analysis of CD8A using rabbit polyclonal CD8A Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CD8A gene (Lane 2).
2 / 3
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CD8A antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot analysis of CD8A using rabbit polyclonal CD8A Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CD8A gene (Lane 2).
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CD8A antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

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PSI-57-512-400ul400ul£626.00
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Further Information

Additional Names:
T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2, CD8a, CD8A, MAL
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50
Background:
The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a co-receptor, and the T-cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional co-receptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.
Background References:
  • Mancebo,E., Mol. Immunol. 45 (2), 479-484 (2008)
  • Zhou,H., Transplant. Proc. 39 (10), 3065-3067 (2007)
  • Borchers,M.T., Exp. Mol. Pathol. 83 (3), 301-310 (2007)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This CD8A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 150-180 amino acids from the C-terminal region of human CD8A.
NCBI Gene ID #:
925
NCBI Official Name:
T-cell surface glycoprotein CD8 alpha chain
NCBI Official Symbol:
CD8A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
26 kDa
Protein Accession #:
P01732
Protein GI Number:
116035
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Immunology,Signal Transduction
Swissprot #:
P01732
User NOte:
Optimal dilutions for each application to be determined by the researcher.