ProSci

AGPAT4 Antibody

Product Code:
 
PSI-63-601
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of AGPAT4 Antibody in MDA-MB435 cell line lysates (35ug/lane)
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Flow cytometry analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Western blot analysis of AGPAT4 Antibody in MDA-MB435 cell line lysates (35ug/lane)
Flow cytometry analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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PSI-63-601-400ul400ul£626.00
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Further Information

Additional Names:
1-acyl-sn-glycerol-3-phosphate acyltransferase delta, 1-acylglycerol-3-phosphate O-acyltransferase 4, 1-AGP acyltransferase 4, 1-AGPAT 4, Lysophosphatidic acid acyltransferase delta, LPAAT-delta, AGPAT4
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
AGPAT4 converts lysophosphatidic acid (LPA) into phosphatidic acid by incorporating an acyl moiety at the sn-2 position of the glycerol backbone.
Background References:
  • Leung,D.W. et.al., Front. Biosci. 6, D944-D953 (2001)
  • Clark,H.F., et.al., Genome Res. 13 (10), 2265-2270 (2003)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Monkey
Immunogen:
This AGPAT4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 176-202 amino acids from the Central region of human AGPAT4.
NCBI Gene ID #:
56895
NCBI Official Name:
1-acyl-sn-glycerol-3-phosphate acyltransferase delta
NCBI Official Symbol:
AGPAT4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
44 kDa
Protein Accession #:
Q9NRZ5
Protein GI Number:
12230468
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Obesity,Signal Transduction
Swissprot #:
Q9NRZ5
User NOte:
Optimal dilutions for each application to be determined by the researcher.