ProSci

CA9 Antibody

Product Code:
 
PSI-61-750
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of CA9 antibody in Hela cell line lysates (35ug/lane)
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Flow cytometric analysis of hela cells using CA9 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Western blot analysis of CA9 antibody in Hela cell line lysates (35ug/lane)
Flow cytometric analysis of hela cells using CA9 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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PSI-61-750-400ul400ul£626.00
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Further Information

Additional Names:
Carbonic anhydrase 9, Carbonate dehydratase IX, Carbonic anhydrase IX, CA-IX, CAIX, Membrane antigen MN, P54/58N, Renal cell carcinoma-associated antigen G250, RCC-associated antigen G250, pMW1, CA9, G250, MN
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50

For IF starting dilution is: 1:10~50
Background:
Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and the only tumor-associated carbonic anhydrase isoenzyme known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation.
Background References:
  • Grabmaier, K., et al., Oncogene 23(33):5624-5631 (2004).
  • Kaluzova, M., et al., Mol. Cell. Biol. 24(13):5757-5766 (2004).
  • Span, P.N., et al., Br. J. Cancer 89(2):271-276 (2003).
  • Hedley, D., et al., Clin. Cancer Res. 9(15):5666-5674 (2003).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This CA9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 113-143 amino acids from the Central region of human CA9.
NCBI Gene ID #:
768
NCBI Official Name:
Carbonic anhydrase 9
NCBI Official Symbol:
CA9
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
50 kDa
Protein Accession #:
Q16790
Protein GI Number:
83300925
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer,Obesity,Signal Transduction
Swissprot #:
Q16790
User NOte:
Optimal dilutions for each application to be determined by the researcher.