ProSci

CPT1B Antibody

Product Code:
 
PSI-61-417
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
1 / 2
Western blot analysis of CPT1B using rabbit polyclonal CPT1B Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CPT1B gene (Lane 2).
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Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
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Western blot analysis of CPT1B using rabbit polyclonal CPT1B Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CPT1B gene (Lane 2).
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.

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PSI-61-417-400ul400ul£626.00
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Further Information

Additional Names:
Carnitine O-palmitoyltransferase 1, muscle isoform, CPT1-M, Carnitine O-palmitoyltransferase I, muscle isoform, CPT I, CPTI-M, Carnitine palmitoyltransferase 1B, Carnitine palmitoyltransferase I-like protein, CPT1B, KIAA1670
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
CPT1B is a member of the carnitine/choline acetyltransferase family, and is the rate-controlling enzyme of the long-chain fatty acid beta-oxidation pathway in muscle mitochondria. This enzyme is required for the net transport of long-chain fatty acyl-CoAs from the cytoplasm into the mitochondria.
Background References:
  • Dai, J., et al., Biochem. Biophys. Res. Commun. 301(3):758-763 (2003).
  • van der Leij, F.R., et al., J. Biol. Chem. 277(30):26994-27005 (2002).
  • Hirosawa, M., et al., DNA Res. 8(1):1-9 (2001).
  • Yamazaki, N., et al., J. Biol. Chem. 275(41):31739-31746 (2000).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This CPT1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 606-637 amino acids from the C-terminal region of human CPT1B.
NCBI Gene ID #:
1375
NCBI Official Name:
Carnitine O-palmitoyltransferase 1, muscle isoform
NCBI Official Symbol:
CPT1B
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
88 kDa
Protein Accession #:
Q92523
Protein GI Number:
2493497
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer,Obesity,Signal Transduction
Swissprot #:
Q92523
User NOte:
Optimal dilutions for each application to be determined by the researcher.