ProSci

FZD1 Antibody

Product Code:
 
PSI-61-489
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of anti-FZD1 Antibody in Hela cell line lysates (35ug/lane)
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Flow cytometric analysis of NCI-H292 cells using FZD1 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Western blot analysis of anti-FZD1 Antibody in Hela cell line lysates (35ug/lane)
Flow cytometric analysis of NCI-H292 cells using FZD1 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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PSI-61-489-400ul400ul£626.00
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Further Information

Additional Names:
Frizzled-1, Fz-1, hFz1, FzE1, FZD1
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50

For IHC-P starting dilution is: 1:10~50
Background:
Members of the 'frizzled' family are 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The FZD1 protein contains a signal peptide, a cysteine-rich domain in the N-terminal extracellular region, 7 transmembrane domains, and a C-terminal PDZ domain-binding motif.
Background References:
  • Quelard,D., (er) PLoS ONE(4), E1878 (2008)
  • Hardie,W.D.,Am. J. Respir. Cell Mol. Biol. 37 (3), 309-321 (2007)
  • Yang,L., J. Dermatol. Sci. 42 (2), 111-119 (2006)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This FZD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 367-396 amino acids from the Central region of human FZD1.
NCBI Gene ID #:
8321
NCBI Official Name:
Frizzled-1
NCBI Official Symbol:
FZD1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
71 kDa
Protein Accession #:
Q9UP38
Protein GI Number:
92058705
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer,Signal Transduction,Growth Factor
Swissprot #:
Q9UP38
User NOte:
Optimal dilutions for each application to be determined by the researcher.