ProSci

NCAPG2 Antibody

Product Code:
 
PSI-58-920
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
1 / 2
Western Blot at 1:1000 dilution + MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.
2 / 2
Western blot analysis in 293 cell line lysates (35ug/lane).
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Western Blot at 1:1000 dilution + MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in 293 cell line lysates (35ug/lane).

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PSI-58-920-400ul400ul£626.00
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Further Information

Additional Names:
Condensin-2 complex subunit G2, Chromosome-associated protein G2, CAP-G2, hCAP-G2, Leucine zipper protein 5, Non-SMC condensin II complex subunit G2, NCAPG2, LUZP5
Application Note:
For WB starting dilution is: 1:1000
Background:
Condensin complexes I and II play essential roles in mitotic chromosome assembly and segregation. Both condensins contain 2 invariant structural maintenance of chromosome (SMC) subunits, SMC2 (MIM 605576) and SMC4 (MIM 605575), but they contain different sets of non-SMC subunits. NCAPG2 is 1 of 3 non-SMC subunits that define condensin II (Ono et al., 2003 [PubMed 14532007]).
Background References:
  • Wood, J.L., et al. J. Biol. Chem. 283(43):29586-29592(2008)
  • Nousiainen, M., et al. Proc. Natl. Acad. Sci. U.S.A. 103(14):5391-5396(2006)
  • Nousiainen, M., et al. Proc. Natl. Acad. Sci. U.S.A. 103(14):5391-5396(2006)
  • Smith, E.D., et al. Mol. Cell. Biol. 24(3):1168-1173(2004)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This NCAPG2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 729-757 amino acids from the Central region of human NCAPG2.
NCBI Gene ID #:
54892
NCBI Official Name:
Condensin-2 complex subunit G2
NCBI Official Symbol:
NCAPG2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
131 kDa
Protein Accession #:
Q86XI2
Protein GI Number:
74727834
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle
Swissprot #:
Q86XI2
User NOte:
Optimal dilutions for each application to be determined by the researcher.