ProSci

PITX2 Antibody

Product Code:
 
PSI-57-632
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
1 / 2
Fluorescent image of Hela cell stained with PITX2 Antibody .Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PITX2 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml).PITX2 immunoreactivity is localized to Nucleus significantly.
2 / 2
Western blot analysis of anti-Pilx2 Pab in CEM cell line lysates (35ug/lane).
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Fluorescent image of Hela cell stained with PITX2 Antibody .Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PITX2 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml).PITX2 immunoreactivity is localized to Nucleus significantly.
Western blot analysis of anti-Pilx2 Pab in CEM cell line lysates (35ug/lane).

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PSI-57-632-400ul400ul£626.00
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Further Information

Additional Names:
Pituitary homeobox 2, ALL1-responsive protein ARP1, Homeobox protein PITX2, Paired-like homeodomain transcription factor 2, RIEG bicoid-related homeobox transcription factor, Solurshin, PITX2, ARP1, RGS, RIEG, RIEG1
Application Note:
For IF starting dilution is: 1:10~50

For WB starting dilution is: 1:1000
Background:
Pilx2 is a member of the RIEG/PITX homeobox family, which is in the bicoid class of homeodomain proteins. This protein acts as a transcription factor and regulates procollagen lysyl hydroxylase gene expression. It plays a role in the terminal differentiation of somatotroph and lactotroph cell phenotypes, is involved in the development of the eye, tooth and abdominal organs, and acts as a transcriptional regulator involved in basal and hormone-regulated activity of prolactin. Mutations in this protein are associated with Axenfeld-Rieger syndrome, iridogoniodysgenesis syndrome, and sporadic cases of Peters anomaly. A similar protein in other vertebrates is involved in the determination of left-right asymmetry during development.
Background References:
  • Engenheiro,E., Clin. Genet. 72 (5), 464-470 (2007)
  • Gudbjartsson,D.F., Nature 448 (7151), 353-357 (2007)
  • Lowry,R.B., Am. J. Med. Genet.143 (11), 1227-1230 (2007)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat, Zebrafish, Chicken, Xenopus
Immunogen:
This PITX2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 122-151 amino acids of human PITX2.
NCBI Gene ID #:
5308
NCBI Official Name:
Pituitary homeobox 2
NCBI Official Symbol:
PITX2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
35 kDa
Protein Accession #:
Q99697
Protein GI Number:
6174907
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle
Swissprot #:
Q99697
User NOte:
Optimal dilutions for each application to be determined by the researcher.