ProSci

USP51 Antibody

Product Code:
 
PSI-61-003
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Fluorescent image of Hela cells stained with USP51 Antibody . Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
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Western blot analysis in HepG2,MCF-7 cell line lysates (35ug/lane).
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Fluorescent image of Hela cells stained with USP51 Antibody . Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
Western blot analysis in HepG2,MCF-7 cell line lysates (35ug/lane).

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PSI-61-003-400ul400ul£626.00
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Further Information

Additional Names:
Ubiquitin carboxyl-terminal hydrolase 51, Deubiquitinating enzyme 51, Ubiquitin thioesterase 51, Ubiquitin-specific-processing protease 51, USP51
Application Note:
For IF starting dilution is: 1:25

For WB starting dilution is: 1:1000
Background References:
  • Quesada V., et al. Biochem. Biophys. Res. Commun. 314:54-62(2004).
  • Ross M.T., et al. Nature 434:325-337(2005).
  • Puente X.S., et al. Genome Res. 14:609-622(2004).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This USP51 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 278-307 amino acids from the Central region of human USP51.
NCBI Gene ID #:
158880
NCBI Official Name:
Ubiquitin carboxyl-terminal hydrolase 51
NCBI Official Symbol:
USP51
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
80 kDa
Protein Accession #:
Q70EK9
Protein GI Number:
52000873
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle
Swissprot #:
Q70EK9
User NOte:
Optimal dilutions for each application to be determined by the researcher.