ProSci

SPAG1 Antibody

Product Code:
 
PSI-62-012
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis in Jurkat cell line lysates (35ug/lane).
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SPAG1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis in Jurkat cell line lysates (35ug/lane).
SPAG1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

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PSI-62-012-400ul400ul£626.00
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Further Information

Additional Names:
Sperm-associated antigen 1, HSD-38, Infertility-related sperm protein Spag-1, SPAG1
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
The correlation of anti-sperm antibodies with cases of unexplained infertility implicates a role for these antibodies in blocking fertilization. Improved diagnosis and treatment of immunologic infertility, as well as identification of proteins for targeted contraception, are dependent on the identification and characterization of relevant sperm antigens. The protein expressed by this gene is recognized by anti-sperm agglutinating antibodies from an infertile woman. Furthermore, immunization of female rats with the recombinant human protein reduced fertility. This protein localizes to the plasma membrane of germ cells in the testis and to the post-acrosomal plasma membrane of mature spermatozoa. Recombinant polypeptide binds GTP and exhibits GTPase activity. Thus, this protein may regulate GTP signal transduction pathways involved in spermatogenesis and fertilization. Two transcript variants of this gene encode the same protein. [provided by RefSeq].
Background References:
  • Lin, W., et al. Mol. Hum. Reprod. 7(9):811-818(2001)
  • Diekman, A.B., et al. Am. J. Reprod. Immunol. 37(1):111-117(1997)
  • Zhang, M.L., et al. Chin. Med. J. 105(12):998-1003(1992)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This SPAG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 124-152 amino acids from the N-terminal region of human SPAG1.
NCBI Gene ID #:
6674
NCBI Official Name:
Sperm-associated antigen 1
NCBI Official Symbol:
SPAG1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
104 kDa
Protein Accession #:
Q07617
Protein GI Number:
223634718
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle
Swissprot #:
Q07617
User NOte:
Optimal dilutions for each application to be determined by the researcher.