ProSci

S100A1 Antibody

Product Code:
 
PSI-56-036
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis in MDA-MB435 cell line lysates (35ug/lane).
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S100A1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis in MDA-MB435 cell line lysates (35ug/lane).
S100A1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

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PSI-56-036-400ul400ul£626.00
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Further Information

Additional Names:
Protein S100-A1, S-100 protein alpha chain, S-100 protein subunit alpha, S100 calcium-binding protein A1, S100A1, S100A
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in stimulation of Ca2+-induced Ca2+ release, inhibition of microtubule assembly, and inhibition of protein kinase C-mediated phosphorylation. Reduced expression of this protein has been implicated in cardiomyopathies.
Background References:
  • Marlatt, N.M., et al. Protein Expr. Purif. 73(1):58-64(2010)
  • van Dieck, J., et al. FEBS Lett. 584(15):3269-3274(2010)
  • Sviatoha, V., et al. Melanoma Res. 20(2):118-125(2010)
  • DeRycke, M.S., et al. Am. J. Clin. Pathol. 132(6):846-856(2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This S100A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 45-75 amino acids from the C-terminal region of human S100A1.
NCBI Gene ID #:
6271
NCBI Official Name:
Protein S100-A1
NCBI Official Symbol:
S100A1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
11 kDa
Protein Accession #:
P23297
Protein GI Number:
134136
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle,Neuroscience,Signal Transduction
Swissprot #:
P23297
User NOte:
Optimal dilutions for each application to be determined by the researcher.