Antibody Isotype:
Rabbit Ig
Antibody Clonality:
Polyclonal
Applications:- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
1 / 2
![Western blot analysis of anti-PDXK in HepG2 cell line lysate (35ug/lane) Western blot analysis of anti-PDXK in HepG2 cell line lysate (35ug/lane)](https://www.prosci-inc.com/static-images/62-699_1.jpg)
2 / 2
![Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma. Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.](https://www.prosci-inc.com/static-images/62-699_2.jpg)
❮❯No additional charges, what you see is what you pay! *
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available!
Contact us to find what you can save.
This product comes from:
United States.
Typical lead time:
14-21 working days.
Contact us for more accurate information.
- Further Information
- Documents
- Show All
Further Information
Pyridoxal kinase, Pyridoxine kinase, PDXK, C21orf124, C21orf97, PKH, PNK
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Pyridoxal kinase (PDXK) converts vitamin B6 to pyridoxal-5-phosphate (PLP), an essential cofactor in the intermediate metabolism of amino acids and neurotransmitters. The PDXK gene encodes a 312-amino acid polypeptide, and expression of the cDNA reveals pyridoxal kinase activity. Northern blot analysis revealed that a major 1.5-kb PDXK transcript is expressed in all tissues tested. The expression of PDXK shows circadian oscillations. The expression of Pdxk in mouse liver and brain is regulated by the 3 PAR bZIP transcription factors, Dbp, Hlf, and Tef, which also show circadian oscillations in expression. Mice devoid of all 3 transcription factors show decreased levels of brain PLP, serotonin, and dopamine, and are highly susceptible to frequently lethal generalized spontaneous and audiogenic epilepsies.
- Shin, J.H., et al., Neurochem. Int. 45(1):73-79 (2004).
- Lee, H.S., et al., Mol. Cells 10(4):452-459 (2000).
- Laine-Cessac, P., et al., Biochem. Pharmacol. 54(8):863-870 (1997).
- Hanna, M.C., et al., J. Biol. Chem. 272(16):10756-10760 (1997).
Supplied in PBS with 0.09% (W/V) sodium azide.
batch dependent
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
This PDXK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 242-272 amino acids from the C-terminal region of human PDXK.
8566
Pyridoxal kinase
PDXK
Homo sapiens
Liquid
PREDICTED MOLECULAR WEIGHT:
35 kDa
O00764
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Cell Cycle,Obesity,Signal Transduction
O00764
Optimal dilutions for each application to be determined by the researcher.