ProSci

CLIC4 Antibody

Product Code:
 
PSI-62-994
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
1 / 2
Western blot analysis of CLIC4 using rabbit polyclonal CLIC4 Antibody.293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CLIC4 gene (Lane 2).
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Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with CLIC4 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
❮❯

Western blot analysis of CLIC4 using rabbit polyclonal CLIC4 Antibody.293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CLIC4 gene (Lane 2).
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with CLIC4 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

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PSI-62-994-400ul400ul£626.00
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Further Information

Additional Names:
Chloride intracellular channel protein 4, Intracellular chloride ion channel protein p64H1, CLIC4
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. Chloride intracellular channel 4 (CLIC4) protein, encoded by the CLIC4 gene, is a member of the p64 family; the gene is expressed in many tissues and exhibits a intracellular vesicular pattern in Panc-1 cells (pancreatic cancer cells).
Background References:
  • Singh,H.,FEBS J. 274 (24), 6306-6316 (2007)
  • Suh,K.S.,J. Cell. Sci. 120 (PT 15), 2631-2640 (2007)
  • Suh,K.S.,Clin. Cancer Res. 13 (1), 121-131 (2007)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This CLIC4 antibody is generated from rabbits immunized with recombinant human CLIC4 protein.
NCBI Gene ID #:
25932
NCBI Official Name:
Chloride intracellular channel protein 4
NCBI Official Symbol:
CLIC4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
29 kDa
Protein Accession #:
Q9Y696
Protein GI Number:
20141285
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cell Cycle,Obesity,Signal Transduction
Swissprot #:
Q9Y696
User NOte:
Optimal dilutions for each application to be determined by the researcher.