ProSci

NCF4 Antibody

Product Code:
 
PSI-63-049
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of NCF4 antibody in K562 cell line lysates (35ug/lane)
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Formalin-fixed and paraffin-embedded human lymphoma reacted with NCF4 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot analysis of NCF4 antibody in K562 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human lymphoma reacted with NCF4 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

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PSI-63-049-400ul400ul£626.00
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Further Information

Additional Names:
Neutrophil cytosol factor 4, NCF-4, Neutrophil NADPH oxidase factor 4, SH3 and PX domain-containing protein 4, p40-phox, p40phox, NCF4, SH3PXD4
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
NCF4 is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2(NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity.
Background References:
  • Glas,J., Seiderer,J. Am. J. Gastroenterol. 104 (3), 665-672 (2009)
  • Honbou,K. Seikagaku 80 (8), 743-747 (2008)
  • Dusi,S., Donini,M. Biochem. J. 314 (PT 2), 409-412 (1996)
  • Leto,T.L. Proc. Natl. Acad. Sci. U.S.A. 91 (22), 10650-10654 (1994)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This NCF4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 260-289 amino acids from the C-terminal region of human NCF4.
NCBI Gene ID #:
4689
NCBI Official Name:
Neutrophil cytosol factor 4
NCBI Official Symbol:
NCF4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
39 kDa
Protein Accession #:
Q15080
Protein GI Number:
108884815
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cell Cycle,Obesity,Signal Transduction
Swissprot #:
Q15080
User NOte:
Optimal dilutions for each application to be determined by the researcher.