ProSci

GUSB Antibody

Product Code:
 
PSI-63-905
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of GUSB Antibody in WiDr, A2058, K562 cell line lysates (35ug/lane)
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Formalin-fixed and paraffin-embedded human colon carcinoma reacted with GUSB Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
3 / 3
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Western blot analysis of GUSB Antibody in WiDr, A2058, K562 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human colon carcinoma reacted with GUSB Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

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PSI-63-905-400ul400ul£626.00
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Further Information

Additional Names:
Beta-glucuronidase, Beta-G1, GUSB
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
The GUSB gene encodes beta-glucuronidase (EC 3.2.1.31), a lysosomal hydrolase involved in the stepwise degradation of glucuronic acid-containing glycosaminoglycans (Shipley et al., 1993 [PubMed 7680524]). It is a tetrameric glycoprotein composed of identical subunits (Oshima et al., 1987 [PubMed 3468507]). The GUSB gene is mutated in mucopolysaccharidosis type VII (MPS7; MIM 253220).
Background References:
  • Tomatsu,S. Hum. Mutat. 30 (4), 511-519 (2009)
  • Romanowski,T. Med. Sci. Monit. 14 (7), BR147-BR152 (2008)
  • Gratz,M. Pharmacogenet. Genomics 15 (12), 875-881 (2005)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Pig
Immunogen:
This GUSB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 335-362 amino acids from the Central region of human GUSB.
NCBI Gene ID #:
2990
NCBI Official Name:
Beta-glucuronidase
NCBI Official Symbol:
GUSB
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
75 kDa
Protein Accession #:
P08236
Protein GI Number:
146345377
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle,Obesity,Signal Transduction
Swissprot #:
P08236
User NOte:
Optimal dilutions for each application to be determined by the researcher.