ProSci

CD166 Antibody

Product Code:
 
PSI-61-574
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of CD166 antibody in NCI-H460 cell line lysates (35ug/lane)
2 / 3
Formalin-fixed and paraffin-embedded human brain tissue reacted with CD166 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
3 / 3
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Western blot analysis of CD166 antibody in NCI-H460 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human brain tissue reacted with CD166 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

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PSI-61-574-400ul400ul£626.00
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Further Information

Additional Names:
CD166 antigen, Activated leukocyte cell adhesion molecule, CD166, ALCAM, MEMD
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
CD166 is cell adhesion molecule that binds to CD6. The protein is involved in neurite extension by neurons via heterophilic and homophilic interactions. It may play a role in the binding of T- and B-cells to activated leukocytes, as well as in interactions between cells of the nervous system.
Background References:
  • Kahlert,C., Br. J. Cancer 101 (3), 457-464 (2009)
  • Kulasingam,V., Int. J. Cancer 125 (1), 9-14 (2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Rabbit, Rat
Immunogen:
This CD166 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 58-87 amino acids from the N-terminal region of human CD166.
NCBI Gene ID #:
214
NCBI Official Name:
CD166 antigen
NCBI Official Symbol:
ALCAM
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
65 kDa
Protein Accession #:
Q13740
Protein GI Number:
118572629
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Immunology
Swissprot #:
Q13740
User NOte:
Optimal dilutions for each application to be determined by the researcher.