ProSci

MST3 Antibody

Product Code:
 
PSI-63-290
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of MST3 Antibody in HepG2 cell line lysates (35ug/lane)
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Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.
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Western blot analysis of MST3 Antibody in HepG2 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.

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PSI-63-290-400ul400ul£626.00
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Further Information

Additional Names:
Serine/threonine-protein kinase 24, Mammalian STE20-like protein kinase 3, MST-3, STE20-like kinase MST3, Serine/threonine-protein kinase 24 36 kDa subunit, Mammalian STE20-like protein kinase 3 N-terminal, MST3/N, Serine/threonine-protein kinase 24 12 kDa subunit, Mammalian STE20-like protein kinase 3 C-terminal, MST3/C, STK24, MST3, STK3
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
The yeast 'Sterile 20' gene (STE20) functions upstream of the mitogen-activated protein kinase (MAPK) cascade. In mammals, protein kinases related to STE20 can be divided into 2 subfamilies based on their structure and regulation. Members of the PAK subfamily (see PAK3; MIM 300142) contain a C-terminal catalytic domain and an N-terminal regulatory domain that has a CDC42 (MIM 116952)-binding domain. In contrast, members of the GCK subfamily (see MAP4K2; MIM 603166), also called the Sps1 subfamily, have an N-terminal catalytic domain and a C-terminal regulatory domain without a CDC42-binding domain. STK24 belongs to the GCK subfamily of STE20-like kinases (Zhou et al., 2000 [PubMed 10644707]).[supplied by OMIM]
Background References:
  • Huang, C.Y., et al., J. Biol. Chem. 277(37):34367-34374 (2002).
  • Christian, S.L., et al., Genomics 79(5):635-656 (2002).
  • Zhou, T.H., et al., J. Biol. Chem. 275(4):2513-2519 (2000).
  • Schinkmann, K., et al., J. Biol. Chem. 272(45):28695-28703 (1997).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This MST3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 345-374 amino acids from the C-terminal region of human MST3.
NCBI Gene ID #:
8428
NCBI Official Name:
Serine/threonine-protein kinase 24
NCBI Official Symbol:
STK24
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
49 kDa
Protein Accession #:
Q9Y6E0
Protein GI Number:
13626607
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Neuroscience,Signal Transduction
Swissprot #:
Q9Y6E0
User NOte:
Optimal dilutions for each application to be determined by the researcher.