ProSci

BPGM Antibody

Product Code:
 
PSI-56-539
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western Blot at 1:2000 dilution Lane 1: MDA-MB-453 whole cell lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 ug per lane.
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Western Blot at 1:2000 dilution Lane 1: MDA-MB-453 whole cell lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 ug per lane.

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PSI-56-539-400ul400ul£626.00
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Further Information

Additional Names:
Bisphosphoglycerate mutase, BPGM,3-bisphosphoglycerate mutase, erythrocyte,3-bisphosphoglycerate synthase,3-diphosphoglycerate mutase, DPGM, BPG-dependent PGAM, BPGM
Application Note:
For WB starting dilution is: 1:2000
Background:
2,3-diphosphoglycerate (2,3-DPG) is a small molecule found at high concentrations in red blood cells where it binds to and decreases the oxygen affinity of hemoglobin. This gene encodes a multifunctional enzyme that catalyzes 2,3-DPG synthesis via its synthetase activity, and 2,3-DPG degradation via its phosphatase activity. The enzyme also has phosphoglycerate phosphomutase activity. Deficiency of this enzyme increases the affinity of cells for oxygen. Mutations in this gene result in hemolytic anemia. Multiple alternatively spliced variants, encoding the same protein, have been identified.
Background References:
  • Lamesch, P., et al. Genomics 89(3):307-315(2007)
  • Wang, Y., et al. J. Biol. Chem. 281(51):39642-39648(2006)
  • Wang, Y., et al. J. Biol. Chem. 279(37):39132-39138(2004)
  • Fujita, T., et al. J. Biochem. 124(6):1237-1244(1998)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Monkey
Immunogen:
This BPGM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 230-259 amino acids from the C-terminal region of human BPGM.
NCBI Gene ID #:
669
NCBI Official Name:
Bisphosphoglycerate mutase
NCBI Official Symbol:
BPGM
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
30 kDa
Protein Accession #:
P07738
Protein GI Number:
130350
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Obesity,Signal Transduction
Swissprot #:
P07738
User NOte:
Optimal dilutions for each application to be determined by the researcher.