ProSci

AASS Antibody

Product Code:
 
PSI-55-621
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis in K562 cell line lysates (35ug/lane).
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Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Western blot analysis in K562 cell line lysates (35ug/lane).
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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PSI-55-621-400ul400ul£626.00
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Further Information

Additional Names:
Alpha-aminoadipic semialdehyde synthase, mitochondrial, LKR/SDH, Lysine ketoglutarate reductase, LKR, LOR, Saccharopine dehydrogenase, SDH, AASS
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
This gene encodes a bifunctional enzyme that catalyzes the first two steps in the mammalian lysine degradation pathway. The N-terminal and the C-terminal portions of this enzyme contain lysine-ketoglutarate reductase and saccharopine dehydrogenase activity, respectively, resulting in the conversion of lysine to alpha-aminoadipic semialdehyde. Mutations in this gene are associated with familial hyperlysinemia.
Background References:
  • Sacksteder, K.A., et al. Am. J. Hum. Genet. 66(6):1736-1743(2000)
  • Papes, F., et al. Biochem. J. 344 PT 2, 555-563 (1999) :
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This AASS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 805-834 amino acids from the C-terminal region of human AASS.
NCBI Gene ID #:
10157
NCBI Official Name:
Alpha-aminoadipic semialdehyde synthase, mitochondrial
NCBI Official Symbol:
AASS
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
102 kDa
Protein Accession #:
Q9UDR5
Protein GI Number:
46396032
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Obesity,Signal Transduction
Swissprot #:
Q9UDR5
User NOte:
Optimal dilutions for each application to be determined by the researcher.